Staphylococcus Aureus iyo Staphylococcus Aureus u adkaysta Methicillin
Magaca alaabta
HWTS-OT062 Staphylococcus Aureus iyo Methicillin-Resistant Staphylococcus Aureus Qalabka Ogaanshaha Ashitada Nucleic (Fluorescence PCR)
Shahaado
CE
Epidemiology
Staphylococcus aureus waa mid ka mid ah bakteeriyada pathogenic ee ugu muhiimsan ee caabuqa nosocomial.Staphylococcus aureus (SA) waxay ka tirsan tahay staphylococcus waxayna wakiil ka tahay bakteeriyada Gram-positive, taas oo soo saari karta noocyo kala duwan oo sun ah iyo enzymes soo jiidasho leh.Bakteeriyadu waxay leedahay sifooyinka qaybinta ballaaran, cudur-sidaha xooggan iyo heerka caabbinta sare.Hidda-wadaha Thermostable nuclease (nuc) waa hidde-sidaha aadka loo ilaaliyo ee staphylococcus aureus.
Kanaalka
| FAM | hiddo-wadaha methicillin u adkaysta |
| ROX | Xakamaynta Gudaha |
| CY5 | hiddo-wadaha staphylococcus aureus nuc |
Qiyaasaha Farsamada
| Kaydinta | ≤-18℃ & iftiinka laga ilaaliyo |
| Nolosha shelf | 12 bilood |
| Nooca Tuunada | shaybaarrada caabuqa xaakada, maqaarka iyo unugyada jilicsan, iyo shaybaarka suufka sanka |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/mL staphylococcus aureus, 1000 CFU/mL bakteeriyada u adkaysata methicillin.Marka qalabku ogaado tixraaca LoD ee qaranka, 1000/mL staphylococcus aureus ayaa la ogaan karaa |
| Gaar ahaan | Tijaabada falcelinta iskutallaabtu waxay muujinaysaa in qalabkani aanu lahayn falcelin iskutallaab ah oo leh cudur-sidaha kale ee neef-mareenka sida methicillin-xasaasiyaha staphylococcus aureus, staphylococcus-xun-xun, methicillin-u adkeysiga staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, kleetobisinia, pseudomonas aeruginosa. Mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Qalabka lagu dabaqi karo | Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah SLAN-96P Nidaamyada PCR-Waqtiga-dhabta ah LightCycler®480 Nidaamka PCR-waqtiga dhabta ah LineGene 9600 Plus-Waqtiga dhabta ah ee PCR Nidaamka ogaanshaha MA-6000-waqtiga-dhabta ah ee cabbirka kulaylka wareegga BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
Doorashada 1.
Makro & Micro-Tijaabada Genomic DNA/RNA Kit (HWTS-3019) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. waxaa loo isticmaali karaa Macro & Micro-Tijaabi otomaatig ah Nucleic Acid Extractor (HWTS-3006C, HWTS- 3006B).Ku dar 200µL oo cusbo ah oo caadi ah qulqulka la warshadeeyay, tallaabooyinka xiga waa in la soo saaraa si waafaqsan tilmaamaha, iyo mugga sare u qaadida waa 80µL.
Doorashada 2.
Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ku dar 1mL oo cusbo ah oo caadi ah qulqulka ka dib markaad ku dhaqdo milix caadi ah, ka dibna si fiican isku walaaq.Centrifuge at 13,000r/daqiiqo 5 daqiiqo, ka saar supernatant (ka dhig 10-20µL ee supernatant), oo raac tilmaamaha soo saarista ku xigta.
Soo saarista lagu taliyay: Soo saarista Asiidhka Nucleic ama Reagent Nadiifinta (YDP302) ee Tiangen Biotech (Beijing) Co., Ltd.Soo saarista waa in si adag loo fuliyaa si waafaqsan tillaabada 2 ee buug-tilmaameedka.Waxaa lagu talinayaa in loo isticmaalo RNase iyo biyaha aan DNA-da lahayn si loo iftiimiyo mugga 100µL.
