Fayraska Zika
Magaca alaabta
HWTS-FE002 Xirmada ogaanshaha Ashitada Nukliyeerka ee Zika Virus (Fluorescence PCR)
Shahaado
CE
Epidemiology
Fayraska Zika waxaa iska leh genus Flaviviridae, waa fayraska RNA ee togan ku xidhan, dhexroorkiisuna yahay 40-70nm.Waxay leedahay baqshad, oo ka kooban 10794 nucleotide, oo ay ku dhejiso 3419 amino acids.Marka loo eego genotype, waxa loo qaybiyaa nooca Afrika iyo nooca Aasiya.Cudurka fayraska Zika waa cudur faafa oo ba'an oo is-xakameeya oo ay keento fayraska Zika, kaas oo inta badan lagu kala qaado qaniinyada kaneecada Aedes aegypti.Astaamaha caafimaadku waa inta badan qandho, finan, arthralgia ama conjunctivitis, waana dhif dhimasho.Sida laga soo xigtay Ururka Caafimaadka Adduunka, microcephaly dhallaanka iyo Guillain-Barre syndrome (Guillain-Barré syndrome) ayaa laga yaabaa inay la xiriiraan caabuqa fayraska Zika.
Kanaalka
| FAM | Zika virus nucleic acid |
| ROX | Xakamaynta Gudaha |
Qiyaasaha Farsamada
| Kaydinta | ≤30℃ & iftiinka laga ilaaliyo |
| Nolosha shelf | 12 bilood |
| Nooca Tuunada | serum cusub |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500ng/μL |
| Gaar ahaan | Natiijooyinka baaritaanka ee xirmadani ma saameyn doonto hemoglobin (<800g/L), bilirubin (<700μmol/L), iyo dufannada dhiigga/ triglycerides (<7mmol/L) ee dhiigga. |
| Qalabka lagu dabaqi karo | ABI 7500 Nidaamyada PCR-waqtiga-dhabta ah ABI 7500 Nidaamyada PCR-Waqtiga-Dhakhsada leh QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah SLAN-96P Nidaamyada PCR-Waqtiga-dhabta ah LightCycler®480 Nidaamka PCR-waqtiga dhabta ah LineGene 9600 Plus-Waqtiga dhabta ah ee PCR Nidaamka ogaanshaha MA-6000-waqtiga-dhabta ah ee cabbirka kulaylka wareegga BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah, BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
Doorashada 1.
QIAamp Viral RNA Mini Kit(52904), Soo saarista Asiidhka Nukliyeerka ah ama NadiifintaYDP315-R) ee Tiangen Biotech(Beijing) Co., Ltd.Soo saaristawaa in la soo saaraa iyadoo la raacayo tilmaamaha soo saarista, iyo mugga soo saarista lagu taliyey waa 140 μL iyo mugga sare ee lagu taliyey waa 60 μL.
Doorashada 2.
Makro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) iyo Macro & Micro-Tijaabada Toomaatiga ah ee Ashitada Nukliyeerka (HWTS-3006).Soo saarista waa in lagu soo saaraa si waafaqsan tilmaamaha.Mugga muunadda soo saariddu waa 200 μL, mugga elutionka ee lagu taliyeyna waa 80μL.
