Mycobacterium tuberculosis Nucleic Acid iyo Rifampicin Resistance
Magaca alaabta
HWTS-RT074B-Mycobacterium Tuberculosis Nucleic Acid iyo Rifampicin Resistance Resistance Kit (qallooca dhalaalay)
Shahaado
CE
Epidemiology
Mycobacterium tuberculosis, wax yar sida Qaaxada Bacillus, TB, waa bakteeriyada keenta cudurka qaaxada.Waqtigan xaadirka ah, daawooyinka sida caadiga ah loo isticmaalo safka koowaad ee ka hortagga qaaxada waxaa ka mid ah isoniazid, rifampicin iyo hexambutol, iwm. Si kastaba ha ahaatee, iyadoo ay ugu wacan tahay isticmaalka khaldan ee dawooyinka ka hortagga qaaxada iyo astaamaha qaab dhismeedka gidaarka unugga ee qaaxada mycobacterium, qaaxada mycobacterium waxay samaysaa caabbinta daawada daawooyinka ka hortagga tiibishada, taasoo caqabado culus ku ah ka hortagga iyo daaweynta qaaxada.
Rifampicin ayaa si weyn loogu isticmaalay daawaynta bukaanada qaaxada sambabada tan iyo dabayaaqadii 1970-meeyadii, waxayna leedahay saameyn weyn.Waxay ahayd doorashada koowaad ee lagu soo gaabiyo daaweynta kemotherabi ee bukaannada qaaxada sambabada.Iska caabbinta Rifampicin waxaa badanaa sababa isbeddelka hidda-wadaha rpoB.Inkasta oo dawooyinka cusub ee ka hortagga qaaxada ay si joogto ah u soo baxayaan, iyo waxtarka bukaan-socodka ee bukaannada qaaxada sambabada ayaa sidoo kale sii waday inay soo roonaato, weli waxaa jira la'aanta daawooyinka ka hortagga qaaxada, iyo ifafaalaha isticmaalka daroogada aan macquul ahayn ee bukaan-socodka waa mid aad u sarreeya.Sida iska cad, qaaxada Mycobacterium ee bukaanada qaba qaaxada sambabada gabi ahaanba lama dili karo waqti ku haboon, taasoo aakhirka keenta heerar kala duwan oo ah caabbinta daawada ee bukaanka jirkiisa, sii dheeraato muddada cudurka, kordhisana khatarta dhimashada bukaanka.
Kanaalka
Kanaalka | Kanaalka iyo Fluorophores | Dib u celinta falcelinta A | Dareen-celinta B | Dib u celinta falcelinta C |
FAM Channel | Wariye: FAM, Quencher: Midna | rpoB 507-514 | rpoB 513-520 | 38KD iyo IS6110 |
Kanaalka CY5 | Wariye: CY5, Quencher: Midna | rpoB 520-527 | rpoB 527-533 | / |
HEX (VIC) Channel | Wariye: HEX (VIC), Quencher: Midna | Xakamaynta gudaha | Xakamaynta gudaha | Xakamaynta gudaha |
Qiyaasaha Farsamada
Kaydinta | ≤-18℃ Madow |
Nolosha shelf | 12 bilood |
Nooca Tuunada | xaako |
CV | ≤5.0% |
LoD | qaaxada mycobacterium 50 bakteeriya/mL nooca duurjoogta u adkaysta rifampicin: 2x103bakteeriyada/ml mutant homozygous: 2x103bakteeriyada/ml |
Gaar ahaan | Waxay ogaataa qaaxada mycobacterium-ka duurjoogta ah iyo goobaha isku-bedelka hiddo-wadaha kale ee iska caabbinta daroogada sida katG 315G>C\A, InhA-15C> T, natiijooyinka baaritaanku waxay muujinayaan wax iska caabin ah oo loo yaqaan 'rifampicin', taas oo macnaheedu yahay inaysan jirin wax falcelin ah. |
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