Mycobacterium tuberculosis Nucleic Acid iyo Rifampicin Resistance

Sharaxaad Gaaban:

Qalabkani waxa uu ku haboon yahay in lagu ogaado tayada DNA-ga ee Mycobacterium tuberculosis DNA-da ee shaybaarada xaakada bini'aadamka ee vitro, iyo sidoo kale isku-bedelka homozygous ee gobolka 507-533 amino acid codon ee hidda-wadaha rpoB ee keena iska caabbinta Mycobacterium tuberculosis rifampicin.


Faahfaahinta Alaabta

Tags Product

Magaca alaabta

HWTS-RT074B-Mycobacterium Tuberculosis Nucleic Acid iyo Rifampicin Resistance Resistance Kit (qallooca dhalaalay)

Shahaado

CE

Epidemiology

Mycobacterium tuberculosis, wax yar sida Qaaxada Bacillus, TB, waa bakteeriyada keenta cudurka qaaxada.Waqtigan xaadirka ah, daawooyinka sida caadiga ah loo isticmaalo safka koowaad ee ka hortagga qaaxada waxaa ka mid ah isoniazid, rifampicin iyo hexambutol, iwm. Si kastaba ha ahaatee, iyadoo ay ugu wacan tahay isticmaalka khaldan ee dawooyinka ka hortagga qaaxada iyo astaamaha qaab dhismeedka gidaarka unugga ee qaaxada mycobacterium, qaaxada mycobacterium waxay samaysaa caabbinta daawada daawooyinka ka hortagga tiibishada, taasoo caqabado culus ku ah ka hortagga iyo daaweynta qaaxada.

Rifampicin ayaa si weyn loogu isticmaalay daawaynta bukaanada qaaxada sambabada tan iyo dabayaaqadii 1970-meeyadii, waxayna leedahay saameyn weyn.Waxay ahayd doorashada koowaad ee lagu soo gaabiyo daaweynta kemotherabi ee bukaannada qaaxada sambabada.Iska caabbinta Rifampicin waxaa badanaa sababa isbeddelka hidda-wadaha rpoB.Inkasta oo dawooyinka cusub ee ka hortagga qaaxada ay si joogto ah u soo baxayaan, iyo waxtarka bukaan-socodka ee bukaannada qaaxada sambabada ayaa sidoo kale sii waday inay soo roonaato, weli waxaa jira la'aanta daawooyinka ka hortagga qaaxada, iyo ifafaalaha isticmaalka daroogada aan macquul ahayn ee bukaan-socodka waa mid aad u sarreeya.Sida iska cad, qaaxada Mycobacterium ee bukaanada qaba qaaxada sambabada gabi ahaanba lama dili karo waqti ku haboon, taasoo aakhirka keenta heerar kala duwan oo ah caabbinta daawada ee bukaanka jirkiisa, sii dheeraato muddada cudurka, kordhisana khatarta dhimashada bukaanka.

Kanaalka

Kanaalka

Kanaalka iyo Fluorophores

Dib u celinta falcelinta A

Dareen-celinta B

Dib u celinta falcelinta C

FAM Channel

Wariye: FAM, Quencher: Midna

rpoB 507-514

rpoB 513-520

38KD iyo IS6110

Kanaalka CY5

Wariye: CY5, Quencher: Midna

rpoB 520-527

rpoB 527-533

/

HEX (VIC) Channel

Wariye: HEX (VIC), Quencher: Midna

Xakamaynta gudaha

Xakamaynta gudaha

Xakamaynta gudaha

Qiyaasaha Farsamada

Kaydinta

≤-18℃ Madow

Nolosha shelf

12 bilood

Nooca Tuunada

xaako

CV

≤5.0%

LoD

qaaxada mycobacterium 50 bakteeriya/mL

nooca duurjoogta u adkaysta rifampicin: 2x103bakteeriyada/ml

mutant homozygous: 2x103bakteeriyada/ml

Gaar ahaan

Waxay ogaataa qaaxada mycobacterium-ka duurjoogta ah iyo goobaha isku-bedelka hiddo-wadaha kale ee iska caabbinta daroogada sida katG 315G>C\A, InhA-15C> T, natiijooyinka baaritaanku waxay muujinayaan wax iska caabin ah oo loo yaqaan 'rifampicin', taas oo macnaheedu yahay inaysan jirin wax falcelin ah.

Qalabka lagu dabaqi karo:

SLAN-96P Nidaamyada PCR-Waqtiga-dhabta ah

BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah

LightCycler480® Nidaamka PCR-waqtiga-dhabta ah

Socodka Shaqada

4697e0586927f02cf6939f68fc30ffc


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