Mycobacterium Tuberculosis INH iska caabinta
Magaca Alaabta
HWTS-RT002A-Mycobacterium Qaaxada Isoniazid Qalabka Ogaanshaha Iska caabinta (Fluorescence PCR)
Epidemiology
Isoniazid, oo ah daawada muhiimka ah ee ka hortagga qaaxada ee la soo bandhigay 1952, waa mid ka mid ah daawooyinka ugu waxtarka badan ee daaweynta la isku daray ee qaaxada firfircoon iyo hal daawo oo loogu talagalay qaaxada qarsoon.
KatG waa hiddo-wadaha ugu weyn ee codeeya catalase-peroxidase iyo isbeddellada hidda-wadaha katG waxay kor u qaadi kartaa isku-darka gidaarka unugyada mycolic acid, taasoo ka dhigaysa bakteeriyada u adkaysata isoniazid.Muujinta KatG waxay si xun ula xiriirtaa isbeddellada INH-MIC, iyo 2-laab hoos u dhaca muujinta katG waxay keenaysaa koror 2-laab ah MIC.Sababta kale ee iska caabbinta isoniazid ee qaaxada mycobacterium waxay dhacdaa marka la geliyo saldhigga, tirtirka ama isbeddelka ay ku dhacdo goobta hidda-wadaha InhA ee qaaxada mycobacterium.
Kanaalka
| ROX | inhA (-15C>T) goobta · |
| CY5 | katG (315G>C) goobta |
| VIC (HEX) | IS6110 |
Qiyaasaha Farsamada
| Kaydinta | ≤-18℃ Madow |
| Nolosha shelf | 12 bilood |
| Nooca Tuunada | xaako |
| CV | ≤5.0% |
| LoD | 1 × 103bakteeriyada/ml |
| Gaar ahaan | falcelin la'aan iskutallaab la'aan ah oo leh isbeddellada afarta goobood ee iska caabbinta daroogada (511, 516, 526 iyo 531) ee hiddo-wadaha rpoB ee ka baxsan tirada ogaanshaha ee xirmada ogaanshaha. Qalabka lagu dabaqi karo: Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah ee degdegga ah QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah SLAN-96P Nidaamyada PCR-Waqtiga-dhabta ah LightCycler®480 Nidaamka PCR-waqtiga-dhabta ah LineGene 9600 Plus-Waqtiga dhabta ah ee PCR Nidaamka ogaanshaha MA-6000-waqtiga-dhabta ah ee cabbirka kulaylka wareegga BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
