Fayraska Papillomavirus (28 Nooc) Qalabka Garashada Genotyping (Fluorescence PCR)

Xirmada ayaa adeegsata hab-oggolaanshaha fluorescence-ka badan ee nucleic acid amplification (PCR).Aasaasayaal aad u gaar ah iyo baarayaal ayaa loo qaabeeyey iyadoo lagu salaynayo hiddasidaha L1 ee taxanaha bartilmaameedka HPV.Baaritaanka gaarka ah waxaa lagu calaamadeeyay FAM fluorophore (HPV6, 16, 26, 40, 53, 58, 73), VIC/HEX fluorophore (HPV11, 18, 33, 43, 51, 59, 81), CY5 fluorophore (H4PV35), , 45, 54, 56, 68, 82) iyo ROX fluorophore (HPV31, 39, 42, 52, 61, 66, 83) at 5', iyo 3' quencher group waa BHQ1 ama BHQ2.Inta lagu jiro kor u qaadida PCR, aasaasiyaal gaar ah iyo baarayaashu waxay ku xidhan yihiin taxanaha bartilmaameedkooda.Marka Taq enzyme uu la kulmo baaritaannada ku xiran taxanaha bartilmaameedka, waxay ka shaqeysaa shaqada 5 'dhammaadka exonuclease si ay u kala saarto weriyaha fluorophore ka fluorophore quencher, si nidaamka kormeerka fluorescence uu helo signalka fluorescent, taas oo ah, mar kasta oo DNA ah. strand waa la xoojiyay, molecule fluorescent ah ayaa la sameeyay, kaas oo ogaanaya is-waafajinta dhammaystiran ee ururinta calaamadaha fluorescent iyo samaynta alaabada PCR, si loo gaaro tayada tayada iyo genotyping ee acids nucleic ee 28 noocyada papillomavirus bini'aadamka ee shaybaarada unugyada ilmo-galeenka .