Bani'aadamka BRAF Hida V600E
Magaca alaabta
HWTS-TM007-Bani'aadamka BRAF Hida V600E Qalabka Ogaanshaha Isbedelka (Fluorescence PCR)
Shahaado
CE
Epidemiology
In ka badan 30 nooc oo isbeddellada BRAF ah ayaa la helay, kuwaas oo qiyaastii 90% ay ku yaalliin exon 15, halkaas oo isbeddelka V600E loo arko inuu yahay isbeddelka ugu badan, taas oo ah, thymine (T) oo ku yaal booska 1799 ee exon 15 ayaa la beddelay adenine (A), taasoo keentay beddelka valine (V) ee booska 600 by glutamic acid (E) ee alaabta borotiinka.Isbeddellada BRAF waxaa caadi ahaan laga helaa burooyinka halista ah sida melanoma, kansarka mindhicirka, kansarka tayroodhka, iyo kansarka sanbabada.Fahamka beddelka hidda-wadaha BRAF waxay noqotay baahida loo qabo in lagu baadho EGFR-TKIs iyo BRAF dawooyinka beddelka hidda-wadaha ee ku jira daawaynta dawada lala beegsaday ee bukaannada laga faa'iidaysan karo.
Kanaalka
| FAM | Isbedelka V600E, xakamaynta gudaha |
Qiyaasaha Farsamada
| Kaydinta | ≤-18℃ |
| Nolosha shelf | 9 bilood |
| Nooca Tuunada | shaybaarada unugyada cudur-sidaha ee paraffin-ku-xidhan |
| CV | 5.0% |
| Ct | ≤38 |
| LoD | Isticmaal qalabka si aad u ogaato xakamaynta tayada LoD ee u dhiganta.a) ka hooseeya 3ng/μL asalka duurjoogta ah, 1% heerka isbeddelka ayaa lagu ogaan karaa falcelinta falcelinta;b) ka hooseeya 1% heerka isku-beddelka, isku-beddelka 1×103Koobiyo/ml ee asalka duurjoogta ah ee 1×105Nuqullada/ml waxaa si adag looga ogaan karaa kaydiyaha falcelinta;c) IC Reaction Buffer wuxuu ogaan karaa xakamaynta tayada ugu hooseysa ee SW3 ee kontoroolka gudaha ee shirkadda. |
| Qalabka lagu dabaqi karo: | Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ahNidaamyada noolaha ee la dabaqay 7300 PCR-waqtiga-dhabta ah Nidaamyada, QuantStudio® 5 Nidaamyada PCR-waqtiga-dhabta ah LightCycler®480 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
Soo saarista lagu taliyay: QIAGEN's QIAamp DNA FFPE Tissue Kit (56404), Paraffin-ku-xidhan Qalab DNA-da Degdegga ah ee Soosaarista (DP330) oo ay soo saartay Tiangen Biotech(Beijing) Co., Ltd.
