15 Noocyada Khatarta Sare ee Human Papillomavirus E6/E7 Gene mRNA
Magaca alaabta
HWTS-CC005A-15 Noocyada Khatarta Sare leh ee Bini'aadamka Papillomavirus E6/E7 Qalabka Ogaanshaha mRNA (Fluorescence PCR)
Epidemiology
Kansarka ilmo-galeenka waa mid ka mid ah noocyada ugu badan ee kansarka dheddigga ee adduunka oo dhan, dhacdadiisuna waxay si dhow ula xiriirtaa papillomaviruses (HPV), laakiin qayb yar oo ka mid ah caabuqyada HPV ayaa isu beddeli kara kansar.Halista sare ee HPV waxa ay ku dhacdaa unugyada epithelial-ka ilmo-galeenka waxayna soo saartaa laba kankorootein, E6 iyo E7.Borootiinkani waxa uu saamayn karaa noocyo kala duwan oo borotiinno gacanta ah (sida borotiinada xakamaynta burooyinka pRB iyo p53), waxay dheereeyaan wareegga unugyada, waxay saameeyaan isku-dhafka DNA-ga iyo xasiloonida genome-ga, waxayna farageliyaan jawaabaha difaaca fayraska iyo antitumor.
Kanaalka
| Kanaalka | Qayb | Genotype waa la tijaabiyay |
| FAM | HPV Reaction Buffer 1 | HPV16,31,33,35,51,52,58 |
| VIC/HEX | hidda-wadaha β-actin aadanaha | |
| FAM | HPV Reaction Buffer 2 | HPV 18,39,45,53,56,59,66,68 |
| VIC/HEX | INS hidda-wadaha |
Qiyaasaha Farsamada
| Kaydinta | Dareeraha: ≤-18℃ |
| Nolosha shelf | 9 bilood |
| Nooca Tuunada | unug exfoliated afka ilmagaleenka |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 koobi/ml |
| Qalabka lagu dabaqi karo | Nidaamyada noolaha ee la dabaqay 7500 Nidaamka PCR-waqtiga-dhabta ah Nidaamyada noolaha ee la dabaqay 7500 Nidaamyada PCR-waqtiga-dhabta ah ee degdegga ah QuantStudio®5 Nidaamyada PCR-waqtiga-dhabta ah SLAN-96P Nidaamyada PCR-Waqtiga-dhabta ah LightCycler®480 Nidaamka PCR-waqtiga-dhabta ah LineGene 9600 Plus-Waqtiga dhabta ah ee PCR Nidaamka ogaanshaha MA-6000-waqtiga-dhabta ah ee cabbirka kulaylka wareegga BioRad CFX96 Nidaamka PCR-waqtiga-dhabta ah BioRad CFX Opus 96 Nidaamka PCR-waqtiga-dhabta ah |
Socodka Shaqada
Soo saarista lagu taliyay: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Soo saarista waa in loo fuliyaa si adag iyadoo la raacayo tilmaamaha isticmaalka .Mugga sare ee lagu taliyey waa 50μL.Haddii muunada aan si buuxda loo dheefshiido, ku celi tallaabada 4 si aad dib u dheefshiidid.Ka dibna tijaabi sida waafaqsan tilmaamaha isticmaalka.
Soo saarista lagu taliyay: RNAprep Pure Animal Tissue Total RNA Extraction Kit (DP431).Soo saarista waa in loo fuliyaa si waafaqsan tilmaamaha isticmaalka si adag (Tallaabada 5, labanlaab xoojinta Xalka shaqada ee DNaseI, taas oo ah, qaado 20μL ee RNase-Free DNaseI (1500U) ee kaydka kaydka tubbada cusub ee RNase-Free centrifuge, ku dar 60μL ee kaydiyaha RDD, oo si tartiib ah u qas).Mugga sare ee lagu taliyey waa 60μL.Haddii muunada aan si buuxda loo dheefshiido, ku celi tallaabada 5 si aad dib u dheefshiidid.Ka dibna tijaabi sida waafaqsan tilmaamaha isticmaalka.
